Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs.

ABSTRACT:

Background

Polypterus senegalus can fully regenerate its pectoral lobed fins, including a complex endoskeleton, with remarkable precision. However, despite the enormous potential of this species for use in medical research, its regeneration mechanisms remain largely unknown.

Methods

To identify the differentially expressed proteins (DEPs) during the early stages of lobed fin regeneration in P. senegalus, we performed a differential proteomic analysis using isobaric tag for relative and absolute quantitation (iTRAQ) approach based quantitative proteome from the pectoral lobed fins at 3 time points. Furthermore, we validated the changes in protein expression with multiple-reaction monitoring (MRM) analysis.

Results

The experiment yielded a total of 3177 pr

SUBMITTER: Lu S 

PROVIDER: S-EPMC6869209 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature