Isolation of senescent cells by iodixanol (OptiPrep) density gradient-based separation.

ABSTRACT:

Objectives

Chemotherapeutic drugs induce senescence in cancer cells but, unlike replicative senescence or oncogene-induced senescence, do so rather inefficiently and depending on DNA damage. A thorough understanding of the biology of chemotherapy-induced senescent cells requires their isolation from a mixed population of adjacent senescent and non-senescent cancer cells.

Materials and methods

We have developed and optimized a rapid iodixanol (OptiPrep)-based gradient centrifugation system to identify, isolate and characterize doxorubicin (DXR)-induced senescent hepatocellular carcinoma (HCC) cells (HepG2 and Huh-7) in vitro.

Results

After cellular exposure to DXR, we used iodixanol gradient-based centrifugation to isolate and re-plate cells on collagen-coated flasks,

SUBMITTER: Kovacovicova K 

PROVIDER: S-EPMC6869531 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature