LncRNA LOXL1-AS1 facilitates the tumorigenesis and stemness of gastric carcinoma via regulation of miR-708-5p/USF1 pathway.

ABSTRACT:

Objectives

As one of the most life-threatening malignancies, gastric cancer is the third contributor of cancer mortalities globally. Increasing studies have proven the regulatory roles of lncRNAs in the development of diverse malignant tumours. But little is known about its function and molecular mechanism in gastric carcinoma.

Materials and methods

RT-qPCR was performed to measure the expression pattern of LOXL1-AS1 in gastric cancer. To ascertain its definite role, CCK-8, EdU, Western blot, transwell and sphere formation assays were adopted. RNA pull-down, RIP, ChIP and luciferase reporter assays were carried out to investigate the molecular mechanism of LOXL1-AS1 in gastric carcinoma.

Results

LOXL1-AS1 was highly expressed in tissues and cells of gastric cancer. Th

SUBMITTER: Sun Q 

PROVIDER: S-EPMC6869681 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature