Quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter.

ABSTRACT: The presynaptic dopamine transporter mediates rapid reuptake of synaptic dopamine. Although cell surface DAT trafficking recently emerged as an important component of DAT regulation, it has not been systematically investigated. Here, we apply our single quantum dot (Qdot) tracking approach to monitor DAT plasma membrane dynamics in several heterologous expression cell hosts with nanometer localization accuracy. We demonstrate that Qdot-tagged DAT proteins exhibited highly heterogeneous membrane diffusivity dependent on the local membrane topography. We also show that Qdot-tagged DATs were localized away from the flat membrane regions and were dynamically retained in the membrane protrusions and cell edges for the duration of imaging. Single quantum dot tracking of wildtype DAT and its conf

SUBMITTER: Kovtun O 

PROVIDER: S-EPMC6872175 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature