Chromatin activity at GWAS loci identifies T cell states driving complex immune diseases.

ABSTRACT: Immune-disease-associated variants are enriched in active chromatin regions of T cells and macrophages. However, whether these variants function in specific cell states is unknown. Here we stimulated T cells and macrophages in the presence of 13 cytokines and profiled active and open chromatin regions. T cell activation induced major chromatin remodeling, while the presence of cytokines fine-tuned the magnitude of changes. We developed a statistical method that accounts for subtle changes in the chromatin landscape to identify SNP enrichment across cell states. Our results point towards the role of immune-disease-associated variants in early rather than late activation of memory CD4⁺ T cells, with modest differences across cytokines. Furthermore, variants associated with inflamm

SUBMITTER: Soskic B 

PROVIDER: S-EPMC6872452 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature