The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli.

ABSTRACT: Rapid modulation of RNA function by endoribonucleases during physiological responses to environmental changes is known to be an effective bacterial biochemical adaptation. We report a molecular mechanism underlying the regulation of enolase (eno) expression by two endoribonucleases, RNase G and RNase III, the expression levels of which are modulated by oxygen availability in Escherichia coli. Analyses of transcriptional eno-cat fusion constructs strongly suggested the existence of cis-acting elements in the eno 5' untranslated region that respond to RNase III and RNase G cellular concentrations. Primer extension and S1 nuclease mapping analyses of eno mRNA in vivo identified three eno mRNA transcripts that are generated in a manner dependent on RNase III expression, one of which was found

SUBMITTER: Lee M 

PROVIDER: S-EPMC6872547 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature