Plasmon-enhanced stimulated Raman scattering microscopy with single-molecule detection sensitivity.

ABSTRACT: Stimulated Raman scattering (SRS) microscopy allows for high-speed label-free chemical imaging of biomedical systems. The imaging sensitivity of SRS microscopy is limited to ~10 mM for endogenous biomolecules. Electronic pre-resonant SRS allows detection of sub-micromolar chromophores. However, label-free SRS detection of single biomolecules having extremely small Raman cross-sections (~10^-30 cm² sr^-1) remains unreachable. Here, we demonstrate plasmon-enhanced stimulated Raman scattering (PESRS) microscopy with single-molecule detection sensitivity. Incorporating pico-Joule laser excitation, background subtraction, and a denoising algorithm, we obtain robust single-pixel SRS spectra exhibiting single-molecule events, verified by using two isotopologues of a

SUBMITTER: Zong C 

PROVIDER: S-EPMC6872561 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature