Ultra-thin fluorocarbon foils optimise multiscale imaging of three-dimensional native and optically cleared specimens.

ABSTRACT: In three-dimensional light microscopy, the heterogeneity of the optical density in a specimen ultimately limits the achievable penetration depth and hence the three-dimensional resolution. The most direct approach to reduce aberrations, improve the contrast and achieve an optimal resolution is to minimise the impact of changes of the refractive index along an optical path. Many implementations of light sheet fluorescence microscopy operate with a large chamber filled with an aqueous immersion medium and a further inner container with the specimen embedded in a possibly entirely different non-aqueous medium. In order to minimise the impact of the latter on the optical quality of the images, we use multi-facetted cuvettes fabricated from vacuum-formed ultra-thin fluorocarbon (FEP) foils. The

SUBMITTER: Hotte K 

PROVIDER: S-EPMC6872575 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature