Secreting-lux/pT-ClyA engineered bacteria suppresses tumor growth via interleukin-1β in two pathways.

ABSTRACT: Engineered Salmonella typhimurium (S.t-ΔpG^lux/pT-ClyA) and attenuated Salmonella typhimurium (SL: Salmonella typhimurium with a defect in the synthesis of guanine 5'-diphosphate-3'-diphosphate) exhibit similar tumor targeting capabilities (Kim et al. in Theranostics 5:1328-1342, 2015; Jiang et al. in Mol Ther 18:635-642, 2013), but S.t-ΔpG^lux/pT-ClyA exerts superior tumor suppressive effects. The aim of this study was to investigate whether S.t-ΔpG^lux/pT-ClyA inhibits colon cancer growth and recurrence by promoting increased IL-1β production. The CT26 tumor mouse model was used, and mice were treated in the following ways: PBS, S.t-ΔpG^{lux/pT-ClyA(+)} + IL-1βAb, SL, S.t-ΔpG^{lux/pT-ClyA(-)}, and S.t-ΔpG^{lux/pT-ClyA(+)}. Dynamic eva

SUBMITTER: Wu Y 

PROVIDER: S-EPMC6872689 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature