Nonhomologous End Joining Is More Important Than Proton Linear Energy Transfer in Dictating Cell Death.

ABSTRACT:

Purpose

This study seeks to identify biological factors that may yield a therapeutic advantage of proton therapy versus photon therapy. Specifically, we address the role of nonhomologous end-joining (NHEJ) and homologous recombination (HR) in the survival of cells in response to clinical photon and proton beams.

Methods and materials

We irradiated HT1080, M059K (DNA-PKcs^+/+), and HCC1937 human cancer cell lines and their isogenic counterparts HT1080-shDNA-PKcs, HT1080-shRAD51^IND, M059J (DNA-PKcs^-/-), and HCC1937-BRCA1 (BRCA1 complemented) to assess cell clonogenic survival and γ-H2AX radiation-induced foci. Cells were irradiated with either clinically relevant photons or 1 of 3 proton linear energy transfer (LET) values.

Results

Our re

SUBMITTER: Bright SJ 

PROVIDER: S-EPMC6872929 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature