Improved Sensitivity in Low-Input Proteomics Using Micropillar Array-Based Chromatography.

ABSTRACT: Capitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nanoliquid chromatography-electrospray ionization-tandem mass spectrometry (nano-LC-ESI-MS/MS) is currently one of the most sensitive analytical technologies for the comprehensive characterization of complex protein samples. However, despite tremendous technological improvements made in the production and the packing of monodisperse spherical particles for nanoflow high-pressure liquid chromatography (HPLC), current state-of-the-art systems still suffer from limits in operation at the maximum potential of the technology. With the recent introduction of the μPAC system, which provides perfectly ordered micropillar array based chromatographic support materials, completely new ch

SUBMITTER: Stadlmann J 

PROVIDER: S-EPMC6873107 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature