The E3 ubiquitin ligase Pib1 regulates effective gluconeogenic shutdown upon glucose availability.

ABSTRACT: Cells use multiple mechanisms to regulate their metabolic states in response to changes in their nutrient environment. One example is the response of cells to glucose. In Saccharomyces cerevisiae growing in glucose-depleted medium, the re-availability of glucose leads to the down-regulation of gluconeogenesis and the activation of glycolysis, leading to "glucose repression." However, our knowledge of the mechanisms mediating the glucose-dependent down-regulation of the gluconeogenic transcription factors is limited. Using the major gluconeogenic transcription factor Rds2 as a candidate, we identify here a novel role for the E3 ubiquitin ligase Pib1 in regulating the stability and degradation of Rds2. Glucose addition to cells growing under glucose limitation results in a rapid ubiqu

SUBMITTER: Vengayil V 

PROVIDER: S-EPMC6873170 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature