A carbohydrate-binding family 48 module enables feruloyl esterase action on polymeric arabinoxylan.

ABSTRACT: Feruloyl esterases (EC 3.1.1.73), belonging to carbohydrate esterase family 1 (CE1), hydrolyze ester bonds between ferulic acid (FA) and arabinose moieties in arabinoxylans. Recently, some CE1 enzymes identified in metagenomics studies have been predicted to contain a family 48 carbohydrate-binding module (CBM48), a CBM family associated with starch binding. Two of these CE1s, wastewater treatment sludge (wts) Fae1A and wtsFae1B isolated from wastewater treatment surplus sludge, have a cognate CBM48 domain and are feruloyl esterases, and wtsFae1A binds arabinoxylan. Here, we show that wtsFae1B also binds to arabinoxylan and that neither binds starch. Surface plasmon resonance analysis revealed that wtsFae1B's K_d for xylohexaose is 14.8 μm and that it does not bind to star

SUBMITTER: Holck J 

PROVIDER: S-EPMC6873179 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature