G65V Substitution in Actin Disturbs Polymerization Leading to Inhibited Cell Elongation in Cotton.

ABSTRACT: The importance of the actin cytoskeleton for proper cell development has been well established in a variety of organisms. Actin protein sequences are highly conserved, and each amino acid residue may be essential for its function. In this study, we report the isolation and characterization of GhLi ₁ from an upland cotton mutant Ligon lintless-1 (Li₁), which harbors the G65V substitution in its encoded actin protein. Li₁ mutants exhibit pleiotropic malformed phenotypes, including dwarf plants, distorted organs, and extremely shortened fibers. Cytological analysis showed that the actin cytoskeleton was disorganized and the abundance of F-actin was decreased in the Li₁ cells. Vesicles were aggregated into patches, and excessive cellulo

SUBMITTER: Sun Y 

PROVIDER: S-EPMC6873290 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature