MetaSPARSim: a 16S rRNA gene sequencing count data simulator.

ABSTRACT:

Background

In the last few years, 16S rRNA gene sequencing (16S rDNA-seq) has seen a surprisingly rapid increase in election rate as a methodology to perform microbial community studies. Despite the considerable popularity of this technique, an exiguous number of specific tools are currently available for proper 16S rDNA-seq count data preprocessing and simulation. Indeed, the great majority of tools have been developed adapting methodologies previously used for bulk RNA-seq data, with poor assessment of their applicability in the metagenomics field. For such tools and the few ones specifically developed for 16S rDNA-seq data, performance assessment is challenging, mainly due to the complex nature of the data and the lack of realistic simulation models. In fact, to the best of our

SUBMITTER: Patuzzi I 

PROVIDER: S-EPMC6873395 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature