Expression profile analysis identifies IER3 to predict overall survival and promote lymph node metastasis in tongue cancer.

ABSTRACT:

Background

Lymph node metastasis is one of the most important factors affecting the prognosis of tongue cancer, and the molecular mechanism regulating lymph node metastasis of tongue cancer is poorly known.

Methods

The gene expression dataset GSE2280 and The Cancer Genome Atlas (TCGA) tongue cancer dataset were downloaded. R software was used to identify the differentially expressed hallmark gene sets and individual genes between metastatic lymph node tissues and primary tongue cancer tissues, and the Kaplan-Meier method was used to evaluate the association with overall survival. The screening and validation of functional genes was performed using western blot, q-PCR, CCK-8, migration and invasion assays, and lymphangiogenesis was examined by using a tube formation assay.

SUBMITTER: Xiao F 

PROVIDER: S-EPMC6873470 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature