Spatial organization of endometrial gene expression at the onset of embryo attachment in pigs.

ABSTRACT:

Background

During the preimplantation phase in the pig, the conceptus trophoblast elongates into a filamentous form and secretes estrogens, interleukin 1 beta 2, interferons, and other signaling molecules before attaching to the uterine epithelium. The processes in the uterine endometrium in response to conceptus signaling are complex. Thus, the objective of this study was to characterize transcriptome changes in porcine endometrium during the time of conceptus attachment considering the specific localization in different endometrial cell types.

Results

Low-input RNA-sequencing was conducted for the main endometrial compartments, luminal epithelium (LE), glandular epithelium (GE), blood vessels (BV), and stroma. Samples were isolated from endometria collected on Day 14 of pr

SUBMITTER: Zeng S 

PROVIDER: S-EPMC6873571 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature