The proliferative effect of cortisol on bovine endometrial epithelial cells.

ABSTRACT:

Background

Bovine endometrial epithelial cells (BEECs) undergo regular regeneration after calving. Elevated cortisol concentrations have been reported in postpartum cattle due to various stresses. However, the effects of the physiological level of cortisol on proliferation in BEECs have not been reported. The aim of this study was to investigate whether cortisol can influence the proliferation properties of BEECs and to clarify the possible underlying mechanism.

Methods

BEECs were treated with different concentrations of cortisol (5, 15 and 30 ng/mL). The mRNA expression of various growth factors was detected by quantitative reverse transcription-polymerase chain reaction (qPCR), progression of the cell cycle in BEECs was measured using flow cytometric analysis, and the acti

SUBMITTER: Dong J 

PROVIDER: S-EPMC6873581 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature