Induction of IL-22 protein and IL-22-producing cells in rainbow trout Oncorhynchus mykiss.

ABSTRACT: IL-22 is a critical cytokine which is involved in modulating tissue responses during inflammation, and is produced mainly by T cells and innate leucocytes. In mammals, IL-22 is a key component in mucosal defences, tissue repair, epithelial cell survival and proliferation. In teleosts, IL-22 has been cloned and studied in several species, and the transcript is highly expressed in mucosal tissues and induced by pathogen associated molecular patterns (PAMPs), suggesting IL-22 also functions as an important component of the innate immune response in fish. To investigate these immune responses further, we have validated and characterised two monoclonal antibodies (mAbs) which were raised against two different peptide immunogens of salmonid IL-22. Our results show that both mAbs specifically rea

SUBMITTER: Hu Y 

PROVIDER: S-EPMC6873780 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature