Systematic Discovery of Endogenous Human Ribonucleoprotein Complexes.

ABSTRACT: RNA-binding proteins (RBPs) play essential roles in biology and are frequently associated with human disease. Although recent studies have systematically identified individual RNA-binding proteins, their higher-order assembly into ribonucleoprotein (RNP) complexes has not been systematically investigated. Here, we describe a proteomics method for systematic identification of RNP complexes in human cells. We identify 1,428 protein complexes that associate with RNA, indicating that more than 20% of known human protein complexes contain RNA. To explore the role of RNA in the assembly of each complex, we identify complexes that dissociate, change composition, or form stable protein-only complexes in the absence of RNA. We use our method to systematically identify cell-type-specific RNA-associa

SUBMITTER: Mallam AL 

PROVIDER: S-EPMC6873818 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature