TNIP1 Inhibits Proliferation And Promotes Apoptosis In Clear Cell Renal Carcinoma Through Targeting C/Ebpβ.

ABSTRACT:

Background/purpose

TNF-α-induced protein 3-interacting protein 1 (TNIP1) is active in various cancers, but its expression and function in renal cell carcinoma (RCC) have not been described. This study investigated the role of TNIP1 in clear cell renal cell carcinomas (ccRCC), which accounts for 75-80% of RCC and has a poor prognosis.

Methods

The expression of TNIP1 in human ccRCC tissues and cells was detected by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR), Western blot (WB), and immunohistochemical (IHC) staining. Cell proliferation was assayed by a cell counting kit (CCK)-8 assay; cell cycle analysis and apoptosis assay were done by flow cytometry.

Results

TNIP1 is downregulated in both ccRCC human tissues and cells. Besides, TNI

SUBMITTER: Yang Y 

PROVIDER: S-EPMC6874165 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature