Measurement of methylated metabolites using Liquid Chromatography-Mass Spectrometry and its biological application.

ABSTRACT: Methylation aberrations play an important role in many metabolic disorders including cancer. Methylated metabolites are direct indicators of metabolic aberrations, and currently, there is no Liquid chromatography - Mass spectrometry (LC-MS) based method available to cover all classes of methylated metabolites at low detection limits. In this study, we have developed a method for the detection of methylated metabolites, and it's biological application. In this approach, we used a HILIC based HPLC with MS to measure methylated organic acids, amino acids, and nucleotides. These metabolites were separated from each other by their hydrophobic interactions and analyzed by targeted metabolomics of single reaction monitoring by positive and negative mode of electrospray ionization. These metabolit

SUBMITTER: Ambati CR 

PROVIDER: S-EPMC6874373 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature