Ontology highlight
ABSTRACT:
SUBMITTER: Chen P
PROVIDER: S-EPMC6874627 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Cytotechnology 20190927 6
Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been investigated (Gutierrez-Granados et al. in Crit Rev Biotechnol 38:918-940, 2018). Chikungunya Virus-like particle (VLP), expressed by DNA-PEI transient transfection, is a representative case study for these difficulties. Clinical materials were produced in shake flasks, but the proces ...[more]