A mass spectrometry guided approach for the identification of novel vaccine candidates in gram-negative pathogens.

ABSTRACT: Vaccination is the most effective method to prevent infectious diseases. However, approaches to identify novel vaccine candidates are commonly laborious and protracted. While surface proteins are suitable vaccine candidates and can elicit antibacterial antibody responses, systematic approaches to define surfomes from gram-negatives have rarely been successful. Here we developed a combined discovery-driven mass spectrometry and computational strategy to identify bacterial vaccine candidates and validate their immunogenicity using a highly prevalent gram-negative pathogen, Helicobacter pylori, as a model organism. We efficiently isolated surface antigens by enzymatic cleavage, with a design of experiment based strategy to experimentally dissect cell surface-exposed from cytosolic proteins. F

SUBMITTER: Hornburg D 

PROVIDER: S-EPMC6874673 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature