Tunnel engineering to accelerate product release for better biomass-degrading abilities in lignocellulolytic enzymes.

ABSTRACT:

Background

For enzymes with buried active sites, transporting substrates/products ligands between active sites and bulk solvent via access tunnels is a key step in the catalytic cycle of these enzymes. Thus, tunnel engineering is becoming a powerful strategy to refine the catalytic properties of these enzymes. The tunnel-like structures have been described in enzymes catalyzing bulky substrates like glycosyl hydrolases, while it is still uncertain whether these structures involved in ligands exchange. Till so far, no studies have been reported on the application of tunnel engineering strategy for optimizing properties of enzymes catalyzing biopolymers.

Results

In this study, xylanase S7-xyl (PDB: 2UWF) with a deep active cleft was chosen as a study model to evaluate the func

SUBMITTER: Lu Z 

PROVIDER: S-EPMC6874815 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature