Hydroxysteroid sulfotransferase 2B1 affects gastric epithelial function and carcinogenesis induced by a carcinogenic agent.

ABSTRACT:

Background

A healthy gastric mucosal epithelium exhibits tumor-suppressive properties. Gastric epithelial cell dysfunction contributes to gastric cancer development. Oxysterols provided from food or cholesterol oxidation in the gastric epithelium may be further sulfated by hydroxysteroid sulfotransferase 2B1 (SULT2B1), which is highly abundant in the gastric epithelium. However, the effects of SULT2B1 on gastric epithelial function and gastric carcinogenesis are unclear.

Methods

A mouse gastric tumor model was established using carcinogenic agent 3-methylcholanthrene (3-MCA). A SULT2B1 deletion (SULT2B1^-/-) human gastric epithelial line GES-1 was constructed by CRISPR/CAS9 genome editing system.

Results

The gastric tumor incidence was higher in the SULT2B1<

SUBMITTER: Hong W 

PROVIDER: S-EPMC6874824 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature