Midbrain microglia mediate a specific immunosuppressive response under inflammatory conditions.

ABSTRACT:

Background

Inflammation is a critical process for the progression of neuronal death in neurodegenerative disorders. Microglia play a central role in neuroinflammation and may affect neuron vulnerability. Next generation sequencing has shown the molecular heterogeneity of microglial cells; however, the variability in their response to pathological inputs remains unknown.

Methods

To determine the effect of an inflammatory stimulus on microglial cells, lipopolysaccharide (LPS) was administered peripherally to mice and the inflammatory status of the cortex, hippocampus, midbrain, and striatum was assessed. Microglial activation and interaction with the immune system were analyzed in single cell suspensions obtained from the different brain regions by fluorescence-activated cell

SUBMITTER: Abellanas MA 

PROVIDER: S-EPMC6874825 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature