Characterizing the invasion of different breast cancer cell lines with distinct E-cadherin status in 3D using a microfluidic system.

ABSTRACT: E-cadherin is a cell-cell adhesion protein that plays a prominent role in cancer invasion. Inactivation of E-cadherin in breast cancer can arise from gene promoter hypermethylation or genetic mutation. Depending on their E-cadherin status, breast cancer cells adopt different morphologies with distinct invasion modes. The tumor microenvironment (TME) can also affect the cell morphology and invasion mode. In this paper, we used a previously developed microfluidic system to quantify the three-dimensional invasion of breast cancer cells with different E-cadherin status, namely MCF-7, CAMA-1 and MDA-MB-231 with wild type, mutated and promoter hypermethylated E-cadherin, respectively. The cells migrated into a stable and reproducible microfibrous polycaprolactone mesh in the chip under a program

SUBMITTER: Eslami Amirabadi H 

PROVIDER: S-EPMC6875428 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature