Multiscale and multimodal structure-function analysis of intervertebral disc degeneration in a rabbit model.

ABSTRACT:

Objectives

The objective of this study was to perform a quantitative analysis of the structural and functional alterations in the intervertebral disc during in vivo degeneration, using emerging tools that enable rigorous assessment from the microscale to the macroscale, as well as to correlate these outcomes with noninvasive, clinically relevant imaging parameters.

Design

Degeneration was induced in a rabbit model by puncturing the annulus fibrosus (AF) with a 16-gauge needle. 2, 4, 8, and 12 weeks following puncture, degenerative changes in the discs were evaluated via magnetic resonance imaging (MRI), whole motion segment biomechanics, atomic force microscopy, histology and polarized light microscopy, immunohistochemistry, biochemical content, and second harmonic generatio

SUBMITTER: Ashinsky BG 

PROVIDER: S-EPMC6875634 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature