Immediate and deferred epigenomic signatures of in vivo neuronal activation in mouse hippocampus.

ABSTRACT: Activity-driven transcription plays an important role in many brain processes, including those underlying memory and epilepsy. Here we combine genetic tagging of nuclei and ribosomes with RNA sequencing, chromatin immunoprecipitation with sequencing, assay for transposase-accessible chromatin using sequencing and Hi-C to investigate transcriptional and chromatin changes occurring in mouse hippocampal excitatory neurons at different time points after synchronous activation during seizure and sparse activation by novel context exploration. The transcriptional burst is associated with an increase in chromatin accessibility of activity-regulated genes and enhancers, de novo binding of activity-regulated transcription factors, augmented promoter-enhancer interactions and the formation of gene l

SUBMITTER: Fernandez-Albert J 

PROVIDER: S-EPMC6875776 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature