DNA methylation patterns in bladder tumors of African American patients point to distinct alterations in xenobiotic metabolism.

ABSTRACT: Racial/ethnic disparities have a significant impact on bladder cancer outcomes with African American patients demonstrating inferior survival over European-American patients. We hypothesized that epigenetic difference in methylation of tumor DNA is an underlying cause of this survival health disparity. We analyzed bladder tumors from African American and European-American patients using reduced representation bisulfite sequencing (RRBS) to annotate differentially methylated DNA regions. Liquid chromatography-mass spectrometry (LC-MS/MS) based metabolomics and flux studies were performed to examine metabolic pathways that showed significant association to the discovered DNA methylation patterns. RRBS analysis showed frequent hypermethylated CpG islands in African American patients. Further

SUBMITTER: Vantaku V 

PROVIDER: S-EPMC6875901 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature