Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host.

ABSTRACT:

Background

Recent metagenomic analyses have revealed dysbiosis of the gut microbiota of ulcerative colitis (UC) patients. However, the impacts of this dysbiosis are not fully understood, particularly at the strain level.

Results

We perform whole-genome shotgun sequencing of fecal DNA extracts from 13 healthy donors and 16 UC and 8 Crohn's disease (CD) patients. The microbiota of UC and CD patients is taxonomically and functionally divergent from that of healthy donors, with E. faecium being the most differentially abundant species between the two microbial communities. Transplantation of feces from UC or CD patients into Il10^-/- mice promotes pathological inflammation and cytokine expression in the mouse colon, although distinct cytokine expression profiles are ob

SUBMITTER: Seishima J 

PROVIDER: S-EPMC6876129 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature