Unintegrated HIV-1 DNAs are loaded with core and linker histones and transcriptionally silenced.

ABSTRACT: Upon delivery into the nucleus of the host cell, linear double-stranded retroviral DNAs are either integrated into the host genome to form the provirus or act as a target of the DNA damage response and become circularized. Little is known about the chromatinization status of the unintegrated retroviral DNAs of the human immunodeficiency virus type 1 (HIV-1). In this study, we used chromatin immunoprecipitation to investigate the nature of unintegrated HIV-1 DNAs and discovered that core histones, the histone variant H3.3, and H1 linker histones are all deposited onto extrachromosomal HIV-1 DNA. We performed a time-course analysis and determined that the loading of core and linker histones occurred early after virus application. H3.3 and H1 linker histones were also found to be loaded onto

SUBMITTER: Geis FK 

PROVIDER: S-EPMC6876238 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature