Mechanism of synergistic actin filament pointed end depolymerization by cyclase-associated protein and cofilin.

ABSTRACT: The ability of cells to generate forces through actin filament turnover was an early adaptation in evolution. While much is known about how actin filaments grow, mechanisms of their disassembly are incompletely understood. The best-characterized actin disassembly factors are the cofilin family proteins, which increase cytoskeletal dynamics by severing actin filaments. However, the mechanism by which severed actin filaments are recycled back to monomeric form has remained enigmatic. We report that cyclase-associated-protein (CAP) works in synergy with cofilin to accelerate actin filament depolymerization by nearly 100-fold. Structural work uncovers the molecular mechanism by which CAP interacts with actin filament pointed end to destabilize the interface between terminal actin subunits, and

SUBMITTER: Kotila T 

PROVIDER: S-EPMC6876575 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature