Lights, camera, path splitter: a new approach for truly simultaneous dual optical mapping of the heart with a single camera.

ABSTRACT: Optical mapping of transmembrane voltage and intracellular calcium is a powerful tool for investigating cardiac physiology and pathophysiology. However, simultaneous dual mapping of two fluorescent probes remains technically challenging. We introduce a novel, easy-to-use approach that requires a path splitter, single camera and excitation light to simultaneously acquire voltage and calcium signals from whole heart preparations, which can be applied to other physiological models - including neurons and isolated cardiomyocytes. Complementary probes were selected that could be excited with a single wavelength light source. Langendorff-perfused hearts (rat, swine) were stained and imaged using a sCMOS camera outfitted with an optical path splitter to simultaneously acquire two emission fields

SUBMITTER: Jaimes R 

PROVIDER: S-EPMC6876868 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature