Clinical Utility of a Fully Automated Microsatellite Instability Test with Minimal Hands-on Time.

ABSTRACT:

Background

Microsatellite instability (MSI) analysis is becoming increasingly important in many types of tumor including colorectal cancer (CRC). The commonly used MSI tests are either time-consuming or labor-intensive. A fully automated MSI test, the Idylla MSI assay, has recently been introduced. However, its diagnostic performance has not been extensively validated in clinical CRC samples.

Methods

We evaluated 133 samples whose MSI status had been rigorously validated by standard polymerase chain reaction (PCR), clinical nextgeneration sequencing (NGS) cancer panel test, or both. We evaluated the diagnostic performance of the Idylla MSI assay in terms of sensitivity, specificity, and positive and negative predictive values, as well as various sample requirements, such as

SUBMITTER: Lee M 

PROVIDER: S-EPMC6877435 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature