Global chromatin conformation differences in the Drosophila dosage compensated chromosome X.

ABSTRACT: In Drosophila melanogaster the single male chromosome X undergoes an average twofold transcriptional upregulation for balancing the transcriptional output between sexes. Previous literature hypothesised that a global change in chromosome structure may accompany this process. However, recent studies based on Hi-C failed to detect these differences. Here we show that global conformational differences are specifically present in the male chromosome X and detectable using Hi-C data on sex-sorted embryos, as well as male and female cell lines, by leveraging custom data analysis solutions. We find the male chromosome X has more mid-/long-range interactions. We also identify differences at structural domain boundaries containing BEAF-32 in conjunction with CP190 or Chromator. Weakening of these d

SUBMITTER: Pal K 

PROVIDER: S-EPMC6877619 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature