Whole-Exome Sequencing Characterized the Landscape of Somatic Mutations and Pathways in Colorectal Cancer Liver Metastasis.

ABSTRACT:

Purpose

Liver metastasis remains the leading cause of cancer-related mortality in colorectal cancer. The mechanism of occurrence and development of liver metastasis from colorectal cancer is unclear.

Methods

The primary tumor tissues and blood samples of 8 patients with liver metastasis of colorectal cancer were collected, followed by nucleic acid extraction and library construction. Whole-exome sequencing was performed to detect the genomic variations. Bioinformatics was used to comprehensively analyze the sequencing data of these samples, including the differences of tumor mutation burden, the characteristics of gene mutations, and signaling pathways.

Results

The results showed that the top three genes with the highest mutation frequency were TP53, APC

SUBMITTER: Feng L 

PROVIDER: S-EPMC6877969 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature