Mood-stabilizers differentially affect housekeeping gene expression in human cells.

ABSTRACT: Recent studies have revealed that antidepressants affect the expression of constitutively expressed "housekeeping genes" commonly used as normalizing reference genes in quantitative polymerase chain reaction (qPCR) experiments. There has yet to be an investigation however on the effects of mood-stabilizers on housekeeping gene stability. The current study utilized lymphoblastoid cell lines (LCLs) derived from patients with mood disorders to investigate the effects of a range of doses of lithium (0, 1, 2 and 5 mM) and sodium valproate (0, 0.06, 0.03 and 0.6 mM) on the stability of 12 housekeeping genes. RNA was extracted from LCLs and qPCR was used to generate cycle threshold (Ct ) values which were input into RefFinder analyses. The study revealed drug-specific effects on housekeeping gene

SUBMITTER: Powell TR 

PROVIDER: S-EPMC6878232 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature