Compositional alterations of gut microbiota in children with primary nephrotic syndrome after initial therapy.

ABSTRACT: BACKGROUND:Primary nephrotic syndrome (PNS) is a common glomerular disease in children. T cell dysfunction plays a crucial role in the pathogenesis of PNS. Moreover, dysbiosis of gut microbiota contributes to immunological disorders. Whether the initial therapy of PNS affects gut microbiota remains an important question. Our study investigated compositional changes of gut microbiota after initial therapy. METHODS:Fecal samples of 20 children with PNS were collected before and after 4-week initial therapy. Total bacteria DNA were extracted and the V3-V4 regions of bacteria 16S ribosomal RNA gene were sequenced. The composition of gut microbiota before and after initial therapy was analyzed by bioinformatics methods. The function of altered gut microbiota was predicted with PICRUSt method. R

SUBMITTER: Kang Y 

PROVIDER: S-EPMC6878711 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature