29-Color Flow Cytometry: Unraveling Human Liver NK Cell Repertoire Diversity.

ABSTRACT: Recent studies have demonstrated extraordinary diversity in peripheral blood human natural killer (NK) cells and have suggested environmental control of receptor expression patterns on distinct subsets of NK cells. However, tissue localization may influence NK cell differentiation to an even higher extent and less is known about the receptor repertoire of human tissue-resident NK cells. Advances in single-cell technologies have allowed higher resolution studies of these cells. Here, the power of high-dimensional flow cytometry was harnessed to unravel the complexity of NK cell repertoire diversity in liver since recent studies had indicated high heterogeneity within liver NK cells. A 29-color flow cytometry panel allowing simultaneous measurement of surface tissue-residency markers, activa

SUBMITTER: Filipovic I 

PROVIDER: S-EPMC6878906 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature