Monitoring Tuberculosis Drug Activity in Live Animals by Near-Infrared Fluorescence Imaging.

ABSTRACT: Worldwide, tuberculosis (TB) is the leading cause of death due to infection with a single pathogenic agent, Mycobacterium tuberculosis In the absence of an effective vaccine, new, more powerful antibiotics are required to halt the growing spread of multidrug-resistant strains and to shorten the duration of TB treatment. However, assessing drug efficacy at the preclinical stage remains a long and fastidious procedure that delays progression of drugs down the pipeline and towards the clinic. In this investigation, we report the construction, optimization and characterization of genetically engineered near-infrared (NIR) fluorescent reporter strains of the pathogens Mycobacterium marinum and Mycobacterium tuberculosis that enable direct visualization of bacteria in infected zebrafish and mice

SUBMITTER: Sommer R 

PROVIDER: S-EPMC6879209 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature