Evaluation of an Indirect ELISA Using Recombinant Arginine Kinase for Serodiagnosis of Psoroptes ovis var. cuniculi Infestation in Rabbits.

ABSTRACT: Psoroptes ovis var. cuniculi is a common ectoparasite of the wild and domestic rabbits worldwide, which causes significant economic losses in commercial rabbit breeding. In China, the diagnosis of rabbits infested with P. ovis var. cuniculi currently relies on detection of clinical signs and Psoroptes mites in skin scrapings by microscopy examination. However, this method is not very efficient in detection of the low mite loads and/or sub-clinical infections. In the present study, we cloned and expressed an arginine kinase homolog gene from P. ovis var. cuniculi (Poc-AK) and used its recombinant protein rPoc-AK to develop an indirect enzyme-linked immunosorbent assay (iELISA) method for diagnosis of P. ovis var. cuniculi infestation in rabbits. The results showed that the rPoc-AK protein w

SUBMITTER: Gu X 

PROVIDER: S-EPMC6879429 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature