Effective reconstruction of functional organotypic kidney spheroid for in vitro nephrotoxicity studies.

ABSTRACT: Stable and reproducible kidney cellular models could accelerate our understanding of diseases, help therapeutics development, and improve nephrotoxicity screenings. Generation of a reproducible in vitro kidney models has been challenging owing to the cellular heterogeneity and structural complexity of the kidney. We generated mixed immortalized cell lines that stably maintained their characteristic expression of renal epithelial progenitor markers for the different lineages of kidney cellular compartments via the BMP7 signaling pathway from a mouse and a human whole kidney. These cells were used to generate functional and matured kidney spheroids containing multiple renal lineages, such as the proximal tubule, loop of Henle, distal tubules, and podocytes, using extracellular matrix and phy

SUBMITTER: Kang HM 

PROVIDER: S-EPMC6879515 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature