New disease-resistant, seedless grapes are developed using embryo rescue and molecular markers.
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ABSTRACT: Improving the operation of embryo rescue technology is a highly efficient way to breed new high-quality cultivars of seedless, disease-resistant grapes when using a stenospermocarpic Vitis vinifera L. (female parents) hybrid with Chinese wild Vitis (male parents). In this study, sampling time had a significant effect on embryo recovery. Four crosses were investigated to improve the embryo rescue efficiency by determining the best sampling time, which was found to be 40, 50, 55, and 57 days after pollination (DAP) for 'Flame seedless' × 'Ruby seedless', 'Kunxiang seedless' × 'Flame seedless', 'Ruby seedless' self-pollination and 'Ruby seedless' × 'Flame seedless', respectively. The highest percentage embryo germination ranged from 60.83 to 89.10% for four cross-combinations wh
SUBMITTER: Li S
PROVIDER: S-EPMC6879691 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
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