Adipocyte-derived Periostin mediates glucocorticoid-induced hepatosteatosis in mice.

ABSTRACT:

Objective

Long-term glucocorticoids (GCs) therapy usually causes many metabolic side effects, including fatty liver. However, the molecular mechanisms remain poorly understood. Herein, we explored the molecular basis of GCs in the development of fatty liver.

Methods

C57BL/6 male mice were injected with Dexamethasone (DEX) while mouse primary hepatocytes (MPHs), HepG2 and Hep1-6 cells were cultured in the presence of DEX. Genes expression in liver tissues and hepatocytes were assessed by quantitative real-time PCR and western blotting, respectively. To explore whether Periostin is involved in the development of GCs-induced fatty liver, wild-type and Periostin knockout mice were treated with DEX or vehicle control. Luciferase reporter and chromatin immunoprecipitation assays w

SUBMITTER: Wan J 

PROVIDER: S-EPMC6880106 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature