Project description:CGH analysis of Drosophila polytene chromosomes. Drosophila polytene chromosomes were isolated from whole salivary glands dissected from roaming third-instar larvae or from ovaries dissected from adult females. Salivary gland has two biological repeats.
Project description:We replaced the endogenous histones of Drosophila melanogaster with either histones containing an H3K9R mutation or histones containing an H4K16R mutation to interrogate direct functions for histone residues in salivary gland endoreplication. We performed genomic DNA sequencing in HWT (Histone Wild Type) control, H3K9R, and H4K16R females. We found that H3K9 promotes under-replication of pericentromeric heterochromatin but not along chromosome arms and H4K16 is dispensable for under-replication.