Advanced aging causes diaphragm functional abnormalities, global proteome remodeling, and loss of mitochondrial cysteine redox flexibility in mice.

ABSTRACT:

Aim

Inspiratory muscle (diaphragm) function declines with age, contributing to exercise intolerance and impaired airway clearance. Studies of diaphragm dysfunction in rodents have focused on moderate aging (~24months); thus, the impact of advanced age on the diaphragm and potential mechanisms of dysfunction are less clear. Therefore, we aimed to define the effects of advanced age on the mechanics, morphology, and global and redox proteome of the diaphragm.

Methods

We studied diaphragm from young (6months) and very old male mice (30months). Diaphragm function was evaluated using isolated muscle bundles. Proteome analyses followed LC-MS/MS processing of diaphragm muscle.

Results

Advanced aging decreased diaphragm peak power by ~35% and maximal isometric specific force b

SUBMITTER: Kelley RC 

PROVIDER: S-EPMC6880408 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature