Accumulation of damaged mitochondria in alveolar macrophages with reduced OXPHOS related gene expression in IPF.

ABSTRACT: BACKGROUND:Impaired mitochondria homeostasis and function are established hallmarks of aging and increasing evidence suggests a link with lung fibrosis. Mitochondria homeostasis may be also affected in alveolar macrophages (AMs) in idiopathic pulmonary fibrosis (IPF). In this study, we used bronchoalveolar lavage (BAL), a tool for both clinical and research purposes, and a rich source of AMs. METHODS:BAL samples were examined from 52 patients with IPF and 19 healthy individuals. Measurements of mitochondria reactive oxygen species (mtROS), mitochondria morphology and related gene expression were performed. Additionally, autophagy and mitophagy levels were analysed. RESULTS:Mitochondria in AMs from IPF patients had prominent morphological defects and impaired transcription paralleled to a s

SUBMITTER: Tsitoura E 

PROVIDER: S-EPMC6880424 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature