The effects of supraphysiological levels of testosterone on neural networks upstream of gonadotropin-releasing hormone neurons.

ABSTRACT:

Objectives

Several pathological conditions are associated with hyper-production of testosterone; however, its impacts are not well understood. Hence, we evaluated the effects of supraphysiological levels of testosterone on gonadotropin-releasing hormone (GnRH) system in the hypothalamus of male rats. Also, we assessed the expression of two excitatory (kisspeptin and neurokinin-B) and two inhibitory (dynorphin and RFamide-related-peptide) neuropeptides upstream of GnRH neurons as possible routes to relay androgen information.

Materials and methods

Gonadectomized (GDX) male rats received single injection of 100, 250 or 500 mg/kg testosterone undecanoate and three weeks later, posterior (PH) and anterior (AH) hypothalamus was dissected for evaluation of target genes using quant

SUBMITTER: Salehi MS 

PROVIDER: S-EPMC6880527 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature